Journal: Cell Death & Disease

Article Title: Matrix stiffness regulates glucose-6-phosphate dehydrogenase expression to mediate sorafenib resistance in hepatocellular carcinoma through the ITGB1-PI3K/AKT pathway

doi: 10.1038/s41419-025-07842-3

Figure Lengend Snippet: A Venn diagram illustrating the overlap between genes related to ECM stiffness and those associated with sorafenib resistance. B Analysis of the top 20 KEGG pathways for genes related to ECM stiffness and sorafenib resistance. C Cross-validation plot for penalty terms. The cross-validation was used to select the optimum value of the regularization parameter ( λ ) in the LASSO regression model. D LASSO coefficient profiles of genes associated with ECM stiffness and sorafenib resistance. A coefficient profile plot was produced against the log( λ ) sequence. A vertical line was drawn at the selected optimal parameter ( λ ), which resulted in 12 nonzero coefficients. E A forest plot of 12 genes associated with ECM stiffness and sorafenib resistance. F Coefficients of these 12 genes. G Real-time qPCR analysis of gene expression related to ECM stiffness under soft (0.5 kPa) or stiff (12 kPa) matrix conditions. H Western blotting analysis of G6PD and AKR1B20 expression under soft (0.5 kPa) or stiff (12 kPa) matrix conditions. β-actin was used as a loading control and quantification data were shown on the right panel. I Representative images of H&E, Collagen I, α-SMA, Masson’s trichrome, Picrosirius red, AKR1B10, and G6PD staining in HCC tissues from patients who exhibited low stiffness and responsiveness to sorafenib and those with high stiffness and resistance to sorafenib. Scale bar, 200 and 500 μm as indicated. J Quantification of collagen I, α-SMA, Masson’s trichrome, and Picrosirius red area between 10 sorafenib-resistant samples with high ECM and 10 sorafenib-sensitive samples. K Association between AKR1B10 and G6PD expression levels and the degree of ECM stiffness in HCC tissue. ns not significance; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The primary antibodies used were as follows: G6PD (Proteintech, 25413–1-AP, 1:500), AKR1B10 (Proteintech, 18252–1-AP, 1:2000), ITGB1 (Proteintech, 12594–1-AP, 1:1000), collagen I (Proteintech, 10176–2-AP, 1:2500), α-SMA (Proteintech, 66009–1-Ig, 1:500), and p-AKT (Ser473, Proteintech, 66444–1-Ig, 1:2000).

Techniques: Biomarker Discovery, Produced, Sequencing, Gene Expression, Western Blot, Expressing, Control, Staining

Journal:

Article Title: Advanced Imaging of Multiple mRNAs in Brain Tissue using a Custom Hyperspectral Imager and Multivariate Curve Resolution

doi: 10.1016/j.jneumeth.2006.08.018

Figure Lengend Snippet: MCR analysis applied to hyperspectral image data from the dorsal hippocampus (CA1). (A) Grayscale mean intensity image. Entire image contains ~50000 fluorescence emission spectra. Cyan line indicates one line of excitation light used to generate the image. Spectra from each of the 222 pixels on this line are acquired simultaneously in the line-imager. (B) Enlargement of the area shown in (A). Within the cyan excitation line, three pixels of differing spectral composition are called out to illustrate the analysis procedure and results. (C) Raw data and analysis results from three example pixels (Pixel 1, Pixel 2 and Pixel 3) along the line of excitation shown in (A) and (B). Pixels are indicated with yellow arrows and numbered. Each plot shows the raw fluorescence emission spectrum obtained at the pixel of corresponding number in (B), as well as the MCR modeled spectrum and the four individual, scaled component spectra as determined by the analysis for that pixel. (D) 222 overlapping fluorescence emission spectra from all pixels along the cyan line shown in (B). (E) MCR extracted component spectra corresponding to the four dyes in the tissue, Sytox green (523 nm, blue trace), Cy3 (565 nm, green trace), Texas Red (615 nm, orange trace), and Cy5 (670 nm, red trace). The extracted component spectra are normalized to unit length in this panel.

Article Snippet: The hyperspectral imager (HSI) used for these studies was developed by Sandia National Laboratories and is a pushbroom (also known as a linescan) spectrum imaging microscope.

Techniques: Fluorescence

Journal:

Article Title: Advanced Imaging of Multiple mRNAs in Brain Tissue using a Custom Hyperspectral Imager and Multivariate Curve Resolution

doi: 10.1016/j.jneumeth.2006.08.018

Figure Lengend Snippet: High resolution hyperspectral images of the dorsal hippocampus (CA1) labeled with 4 dyes having overlapping emission spectra: Sytox green (523 nm), Cy3 (565 nm), Cy5 (670 nm), and Texas Red (615 nm). In addition to identifying the pure component spectra shown in Fig. 1, the MCR analysis generates images that correspond to the concentrations of each of the components. (A) Sytox green labels the nuclei (blue), (B) Cy3 identifies the immediate early gene, Arc (green), (C) Cy5 labels CAMKII (red), and (D) Texas Red labels GAD65/67 (orange). (D) An RGB image constructed from the false-colored individual images. Mean intensity image of the original raw data is shown in Figure 1a. Scale bar = 10 μm.

Article Snippet: The hyperspectral imager (HSI) used for these studies was developed by Sandia National Laboratories and is a pushbroom (also known as a linescan) spectrum imaging microscope.

Techniques: Labeling, Construct

Journal:

Article Title: Advanced Imaging of Multiple mRNAs in Brain Tissue using a Custom Hyperspectral Imager and Multivariate Curve Resolution

doi: 10.1016/j.jneumeth.2006.08.018

Figure Lengend Snippet: Low resolution hyperspectral images of rat brain coronal sections labeled with 4 dyes having overlapping emission spectra: Sytox green (523 nm), Cy3 (565 nm), Cy5 (670 nm), and Texas Red (615 nm). In addition to identifying the pure component spectra shown in Fig. 1, the MCR analysis generates images that correspond to the concentrations of each of the components. (A) Sytox green labels the nuclei, (B) Cy3 identifies the immediate early gene, Arc, (C) Cy5 labels CAMKII, and (D) Texas Red labels GAD65/67. Scale bar = 1.0 mm.

Article Snippet: The hyperspectral imager (HSI) used for these studies was developed by Sandia National Laboratories and is a pushbroom (also known as a linescan) spectrum imaging microscope.

Techniques: Labeling

Journal: Advances in Experimental Medicine and Biology

Article Title: Hyperspectral Imaging of the Hemodynamic and Metabolic States of the Exposed Cortex: Investigating a Commercial Snapshot Solution

doi: 10.1007/978-3-319-91287-5_3

Figure Lengend Snippet: ( a ) Picture of the setup used for the investigation with the hyperspectral snapshot solution, highlighting the major components; ( b ) RGB microscope picture of the exposed cerebral cortex; ( c ) Spectrally averaged image of the same cortex, acquired during normoxic baseline; ( d ) hyperspectral image of the exposed cortex at 650 nm; ( e ) hyperspectral image of the exposed cortex at 750 nm

Article Snippet: A hyperspectral snapshot cameraHyperspectral imaging (HSI) based on Cubert GmbH technology (the S185 FireflEYE [ ]) was chosen for the study, due to its technical characteristics: primarily, the number of wavelength bands (138) and its spectral resolution (~4 to 8 nm) and sampling (4 nm).

Techniques: Microscopy